All publications:

Xin-Xiang Lim, Bo Shu, Shuijun Zhang, Aaron W K Tan, Thiam-Seng Ng, Xin-Ni Lim, Valerie S-Y Chew, Jian Shi, Gavin R Screaton, Shee-Mei Lok*, Ganesh S Anand*. (2021). Human antibody C10 neutralizes by diminishing Zika but enhancing dengue virus dynamics. Cell, 184(25):6067-6080.e13. 
*co-lead contacts/corresponding authors
PMID: 34852238
Guntur Fibriansah, Xin-Ni Lim, Shee-Mei Lok. (2021). Morphological Diversity and Dynamics of Dengue Virus Affecting Antigenicity. Viruses, 13(8):1446. 
PMID: 34452312
Shee-Mei Lok. (2021). An NTD supersite of attack. Cell Host Microbe., 29(5):744-746.
PMID: 33984277
Justin S.G. Ooi, Shee-Mei Lok. (2021). How NS1 Antibodies Prevent Severe Flavivirus Disease. Trends Biochem Sci., S0968-0004(21)00063-3.
PMID: 33895084
Guntur Fibriansah, Elisa X. Y. Lim, Jan K. Marzinek, Thiam-Seng Ng, Joanne L. Tan, Roland G. Huber, Xin-Ni Lim, Valerie S. Y. Chew, Victor A. Kostyuchenko, Jian Shi, Ganesh S. Anand, Peter J. Bond, James E. Crowe Jr., Shee-Mei Lok . (2021). Antibody affinity versus dengue morphology influences neutralization. PLoS Pathog., 17(2):e1009331.
PMID: 33621239
Qun Fei Zhou, Julie M Fox, James T Earnest, Thiam-Seng Ng, Arthur S Kim, Guntur Fibriansah, Victor A Kostyuchenko, Jian Shi, Bo Shu, Michael S Diamond, Shee-Mei Lok. (2020). Structural basis of Chikungunya virus inhibition by monoclonal antibodies. Proc Natl Acad Sci U S A, 117(44):27637-27645.
PMID: 33087569
Seamus R Morrone, Valerie S Y Chew, Xin-Ni Lim, Thiam-Seng Ng, Victor A Kostyuchenko, Shuijun Zhang, Melissa Wirawan, Pau-Ling Chew, Jaime Lee, Joanne L Tan, Jiaqi Wang, Ter Yong Tan, Jian Shi, Gavin Screaton, Marc C Morais, Shee-Mei Lok. (2020). High flavivirus structural plasticity demonstrated by a non-spherical morphological variant. Nature communications, 11(1), 3112.
PMID: 32561757
Ter Yong Tan, Guntur Fibriansah, Shee-Mei Lok. (2020). Capsid protein is central to the birth of flavivirus particles. PLoS pathogens, 16(5), e1008542.
PMID: 32463839
Shuijun Zhang, Thomas Loy, Thiam-Seng Ng, Xin-Ni Lim, Shyn-Yun Valerie Chew, Ter Yong Tan, Meihui Xu, Victor A. Kostyuchenko, Farhana Tukijan, Jian Shi, Katja Fink, Shee-Mei Lok. (2020). A Human Antibody Neutralizes Different Flaviviruses by Using Different Mechanisms. Cell reports, 31(4), 107584.
PMID: 32348755
Ter Yong Tan, Guntur Fibriansah, Victor A. Kostyuchenko, Thiam-Seng Ng, Xin-Xiang Lim, Shuijun Zhang, Xin-Ni Lim, Jiaqi Wang, Jian Shi, Marc C. Morais, Davide Corti, and Shee-Mei Lok. (2020). Capsid protein structure in Zika virus reveals the flavivirus assembly process. Nat Commun. 11(1):895.
PubMed ID: 32060358
Xin-Ni Lim, Chao Shan, Jan K. Marzinek, Hongping Dong, Thiam Seng Ng, Justin S. G. Ooi, Guntur Fibriansah, Jiaqi Wang, Chandra S. Verma, Peter J. Bond, Pei-Yong Shi, Shee-mei Lok. (2019). Molecular basis of dengue virus serotype 2 morphological switch from 29°C to 37°C. (2019). PLoS Pathog. 15(9):e1007996.
PubMed ID: 31536610
Seamus R. Morrone & Shee-Mei Lok. (2019). Structural perspectives of antibody-dependent enhancement of infection of dengue virus. Curr. Opin. Virol., 36:1-8.
PubMed ID: 30844538
Melissa Wirawan, Guntur Fibriansah, Jan K. Marzinek, Xin Xiang Lim, Thiam-Seng Ng, Adelene Y. L. Sim, Qian Zhang, Victor A. Kostyuchenko, Jian Shi, Scott A. Smith, Chandra S Verma, Ganesh Anand, James E. Crowe Jr., Peter J. Bond & Shee-Mei Lok. (2019). Mechanism of enhanced immature dengue virus attachment to endosomal membrane induced by prM antibody. Structure, 27(2):253-267.e8.
Pubmed ID: 30471923
Shee-Mei Lok. (2018). Flat-lying antibody prevents disease enhancement. Nature Immunology, 19(11):1150-1152.
Pubmed ID: 30323339
Shee-Mei Lok. (2018). Inhibiting EBV: The Key to Carefree Smooching. Immunity., 48(4):623-625.
Pubmed ID: 29669245
Felix A. Rey, Shee-Mei Lok. (2018). Common Features of Enveloped Viruses and Implications for Immunogen Design for Next-Generation Vaccines. Cell, 172(6):1319-1334.
Pubmed ID: 29522750
Emily N Gallichotte, Kenneth H Dinnon III, Xin-Ni Lim, Thiam-Seng Ng, Elisa X.Y. Lim, Vineet D Menachery, Shee-Mei Lok*, Ralph S Baric* (2017) CD-loop extension in Zika virus envelope protein key for stability and pathogenesis. The Journal of Infectious Diseases. J. Infect. Dis., jix473.
Pubmed ID: 28968838
*co-corresponding authors.
Jiaqi Wang, Marco Bardelli, Diego A. Espinosa, Mattia Pedotti, Thiam-Seng Ng, Siro Bianchi, Luca Simonelli, Elisa X.Y. Lim, Mathilde Foglierini, Fabrizia Zatta, Stefano Jaconi, Martina Beltramello, Elisabetta Cameroni, Guntur Fibriansah, Jian Shi, Taylor Barca, Isabel Pagani, Alicia Rubio, Vania Broccoli, Elisa Vicenzi, Victoria Graham, Steven Pullan, Stuart Dowall, Roger Hewson, Simon Jurt, Oliver Zerbe, Karin Stettler, Antonio Lanzavecchia, Federica Sallusto, Andrea Cavalli, Eva Harris, Shee-Mei Lok*, Luca Varani*, Davide Corti*. (2017). A human bi-specific antibody against Zika virus with high therapeutic potential. Cell, 171(1):229-241.
Pubmed ID: 28938115
*co-corresponding authors. 
Xin-Xiang Lim, Arun Chandramohan, Xin Ying Elisa Lim, James E. Crowe, Jr., Shee-Mei Lok, Ganesh S. Anand. (2017). Epitope and Paratope Mapping Reveals Temperature-Dependent Alterations in the Dengue-Antibody Interface. Structure, 25(9):1391-1402. 
Pubmed ID: 28823471
Shee-Mei Lok. (2017). Unsweetened IgG is bad for dengue patients. Cell Host and Microbes, 21(3):312-314. 
Pubmed ID: 28279340
Xin-Xiang Lim, Arun Chandramohan, Xin Ying Elisa Lim, Nirmalya Bag, Kamal Kant Sharma, Melissa Wirawan, Thorsten Wohland, Shee-Mei Lok, Ganesh S. Anand. (2017). Conformational changes in intact dengue virus reveal serotype-specific expansion. Nature Communications, 8:14339.
Pubmed ID: 28186093
Shuijun Zhang, Victor A. Kostyuchenko, Thiam-Seng Ng, Xin-Ni Lim, Justin S.G. Ooi, Sebastian Lambert, Ter Yong Tan, Douglas G. Widman, Jian Shi, Ralph S. Baric and Shee-Mei Lok. (2016). Neutralization mechanism of a highly potent antibody against Zika virus. Nature Communications, 7:13679.
Pubmed ID: 27882950
Victor A. Kostyuchenko, Elisa X. Y. Lim, Shuijun Zhang, Guntur Fibriansah, Thiam-Seng Ng, Justin S. G. Ooi, Jian Shi. and Shee-Mei Lok. (2016). Structure of the thermally stable Zika virus. Nature. 533(7603):425-428.
Pubmed ID: 27093288
​Guntur Fibriansah and Shee-Mei Lok. (2016). The development of therapeutic antibodies against dengue virus. Antiviral Research, 128:7-19. 
Pubmed ID: 26794397
Shee-Mei Lok. (2015). The interplay of dengue virus morphological diversity and human antibodies. Trends in Microbiology, 24(4):284-293.
Pubmed ID: 26747581
Guntur Fibriansah, Kristie D. Ibarra, Thiam-Seng Ng, Scott A. Smith, Joanne L. Tan, Xin-Ni Lim, Justin S. G. Ooi, Victor A. Kostyuchenko, Jiaqi Wang, Aravinda M. de Silva, Eva Harris, James E. Crowe, Jr. and Shee-Mei Lok. (2015). DENGUE VIRUS: Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers. Science. 349(6243):88-91.
Pubmed ID: 26138979
#highlighted article in Science, commentary articles on this paper is published in the journals “Nature Reviews Drug Discovery” and “Nature”.
Satoru Watanabe, Kitti Wing Ki Chan, Jiaqi Wang, Laura Rivino, Shee-Mei Lok, and Subhash G. Vasudevan. (2015). Dengue virus infection with highly-neutralizing levels of cross-reactive antibodies cause acute lethal small intestinal pathology without high level of viremia in mice. J Virol., 89(11):5847-5861.
Pubmed: 25787279
Guntur Fibriansah, Joanne L. Tan, Scott A. Smith, Ruklanthi de Alwis, Thiam-Seng Ng, Victor A. Kostyuchenko, Ramesh S. Jadi, Petra Kukkaro, Aravinda M. de Silva, James E. Crowe, Jr. and Shee-Mei Lok. (2015). A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins. Nature Communications, 6:6341.
Pubmed: 25698059
Phat Vinh Dip, Neelagandan Kamariah, Wilson Nartey, Claudia Beushausen, Victor A. Kostyuchenko, Thiam-Seng Ng, Shee-Mei Lok, Wuan Geok Saw, Frank Eisenhaber, Birgit Eisenhaber, and Gerhard Grüber. (2014). Key roles of the Escherichia coli AhpC C-terminus in assembly and catalysis of alkylhydroperoxide reductase, an enzyme essential for the alleviation of oxidative stress. Biochim Biophys Acta. 1837(12):1932-1943.
Pubmed ID: 25193562
Kuan Rong Chan, Eugenia Z. Ong, Hwee Cheng Tan,  Summer Li-Xin Zhang, Qian Zhang, Kin Fai Tang, Nivashini Kaliaperumal, Angeline Pei Chiew Lim, Martin L. Hibberd, Soh Ha Chan, John E. Connolly, Manoj N. Krishnan, Shee Mei Lok, Brendon J. Hanson, Chao-Nan Lin, and Eng Eong Ooi. (2014). Leukocyte immunoglobulin-like receptor B1 is critical for antibody-dependent dengue. PNAS. 111(7):2722-2727.
Pubmed ID: 24550301
Victor A. Kostyuchenko, Pau Ling Chew, Thiam-Seng Ng, and Shee-Mei Lok. (2014). Near-atomic resolution cryo-EM structure of Dengue serotype 4 virus. Journal of Virology, 88(1):477-482.
Pubmed ID: 24155405
#Selected for cover page and highlighted in a review in Current Opinion in Virology
Shee-Mei Lok. (2014). Human antibodies stop dengue virus by jamming its mechanics. PNAS, 111(5):1670-1671.
Pubmed ID: 24443548
Guntur Fibriansah, Joanne L. Tan, Ruklanthi de Alwis, Scott A. Smith, Thiam-Seng Ng, Victor A. Kostyuchenko, Kristie D. Ibarra, Eva Harris, Aravinda de Silva, James E. Crowe, Jr. and Shee-Mei Lok. (2014). A potent anti-dengue human antibody preferentially recognizes the conformation of E protein monomers assembled on the virus surface. EMBO Molecular Medicine. 6(3):358-71.
Pubmed : 24421336
#Selected for cover page
Guntur Fibriansah, Thiam-Seng Ng, Victor A. Kostyuchenko, Jaime Lee, Sumarlin Lee, Jiaqi Wang, and Shee-Mei Lok. (2013). Structural changes in Dengue virus when exposed to a temperature of 37 deg. C. Journal of Virology, 87(13):7585-92.
Pubmed ID: 23637405
#Selected for cover page and the article was featured in a Nature commentary article and also a review in Current Opinion in Virology
David Veesler, Thiam-Seng Ng, Anoop K. Sendamarai, Brian J. Eilers, Lawrence C. Martin, Shee-Mei Lok, Mark J. Young, John E. Johnson and Chi-yu Fu. (2013). Atomic structure of the 75 MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography. PNAS. 110(14):5504-5509.
Pubmed ID: 23520050
Victor A. Kostyuchenko, Qian Zhang, Joanne L. Tan, Thiam-Seng Ng, and Shee-Mei Lok. (2013). Immature and mature Dengue serotype 1 virus structures provide insight into the maturation process. Journal of Virology. 87(13):7700-7707.
Pubmed ID: 23637416
#this paper featured in a review in Trends in Microbiology
Qian Zhang, Cornelia Hunke, Yin-Hoe Yau, Vernon Seow, Sumarlin Lee, Lukas Bahati Tanner, Xue Li Guan, Markus R Wenk, Guntur Fibriansah, Pau Ling Chew, Petra Kukkaro, Goran Biuković, Pei-Yong Shi, Susana Geifman Shochat, Gerhard Grüber and Shee-Mei Lok. (2012). The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation. Journal of Biological Chemistry. 287(48):40525-40534.
Pubmed ID: 23035113
Gang Zou, Petra Kukkaro, Shee-Mei Lok, Jowin K.W. Ng, Grace K. Tan, Brendon J. Hanson, Sylvie Alonso, Paul A. MacAry, Pei-Yong Shi. (2012). Resistance analysis of an antibody that selectively inhibits dengue virus serotype-1. Antiviral Res. 95(3):216-223. 
Pubmed : 22771779
Shee-Mei Lok , Joshua M. Costin , Yancey M. Hrobowski, Andrew R. Hoffmann, Dawne K. Rowe, Petra Kukkaro, Heather Holdaway, Paul Chipman, Krystal A. Fontaine, Michael R. Holbrook, Robert F. Garry, Victor A. Kostyuchenko, William C. Wimley, Sharon Isern, Michael G. Rossmann, Scott F. Michael. (2012). Release of Dengue Virus Genome Induced by a Peptide Inhibitor. PLOS one. 7(11):e50995.
Pubmed ID: 23226444
Ee Ping Teoh, Petra Kukkaro, En Wei Teo, Angeline Lim, Tze Tong Tan, Andy Yip, Wouter Schul, Myint Aung, Victor A. Kostyuchenko, Yee Sin Leo, Soh Ha Chan, Kenneth G.C. Smith, Eng Eong Ooi, D. Michael Kemeny, Grace K. Tan,  Jowin K. W. Ng, Mah Lee Ng, Sylvie Alonso, Pei Yong Shi, Dale Fisher, Brendon Hanson, Shee-Mei Lok* and Paul A. MacAry*. (2012). The Structural Basis for Serotype-Specific Neutralization of Dengue Virus by a Natural Human Antibody. Science Translational Medicine. 4(139):139ra83.
*co-corresponding authors
Pubmed ID: 22723463
Hongping Dong, David C. Chang, Maggie Ho Chia Hua, Siew Pheng Lim, Yok Hian Chionh, Fabian Hia, Yie Hou Lee, Petra Kukkaro, Shee-Mei Lok, Peter C. Dedon, and Pei-Yong Shi. (2012). 2’-O Methylation of Internal Adenosine by Flavivirus NS5 Methyltransferase. PLOS Pathogen. 8(4):e1002642.
Pubmed ID: 22496660
Annie Hoi Yi Chan, Hwee Ching Tan, Angelia Yee Chow, Angeline Pei Chiew Lim, Shee-Mei Lok, Nikki Moreland, Subhash G. Vasudevan, Paul A. MacAry, Eng Eong Ooi, Brendon J. Hanson. (2012). A human PrM antibody that recognizes a novel cryptic epitope on dengue E glycoprotein. PLOS One. 7(4):e33451
Pubmed ID: 22509258
 Victor A. Kostyuchenko, Joanita Jakana, Xiangan Liu, Andrew D. Haddow, Myint Aung, Scott C. Weaver, Wah Chiu*, and Shee-Mei Lok*. (2011). The structure of barmah forest virus as revealed by cryo-electron microscopy at a 6-angstrom resolution has detailed transmembrane protein architecture and interactions. J Virol. 85(18):9327-33.
*co-corresponding authors
Pubmed ID: 21752915
Joshua M. Costin, Ekachai Jenwitheesuk, Shee-Mei Lok, Elizabeth Hunsperger, Kelly A. Conrads, Krystal A. Fontaine, Craig R. Rees, Michael G. Rossmann, Sharon Isern, Ram Samudrala , Scott F. Michael. (2010). Structural Optimization and De Novo Design of Dengue Virus Entry Inhibitory Peptides. PLOS Neglected Tropical Diseases. 22:721.
Pubmed ID: 20582308
Mickaël V. Cherrier, Bärbel Kaufmann, Grant E. Nybakken, Shee‐Mei Lok, Julia T. Warren, Beverly R. Chen, Christopher A. Nelson, Victor A. Kostyuchenko, Heather A. Holdaway, Paul R. Chipman, Richard J. Kuhn, Michael S. Diamond, Michael G. Rossmann, Daved H. Fremont. (2009). Structural basis for the preferential recognition of immature flaviviruses by a fusion-loop antibody. EMBO J. 28: 3269-3272.
Pubmed ID: 19713934
Long Li, Shee-Mei Lok, I-Mei Yu, Ying Zhang, Richard J. Kuhn, Jue Chen, Michael G. Rossmann. (2008). The flavivirus precursor membrane-envelope protein complex: structure and maturation. Science. 319: 1830-1834.
Pubmed ID: 18369147
Shee-Mei Lok, Victor Kostyuchenko, Grant E. Nybakken, Heather A. Holdaway, Anthony J. Battisti, Soila Sukupolvi-Petty, Dagmar Sedlak, Daved H. Fremont, Paul R. Chipman, John T. Roehrig, Michael S. Diamond, Richard J. Kuhn & Michael G. Rossmann. (2008). Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins. Nature Structural and Molecular Biology. 15 (3): 312-317.
Pubmed ID: 18264114
Won-Suk Chang, Hao Shang, Rushika M. Perera, Shee-Mei Lok, Dagmar Sedlak, Richard J. Kuhn and Gil U Lee. (2008). Rapid detection of dengue virus in serum using Magnetic separation and fluorescence detection. The Analyst.132(2): 233-240.
Pubmed ID: 18227947
Shee-Mei Lok, Mah Lee Ng, John Aaskov. (2001). Amino acid and phenotypic changes in dengue 2 virus associated with escape from neutralization by IgM antibody. J. Med Virol. 65(2): 315-323.
Pubmed ID: 11536239

Book chapters:

Julien Lescar and Shee-Mei Lok. (2014). The structural biology of dengue virus. Chapter 19 in: Dengue and Dengue Hemorrhagic Fever, 2nd Edition; Gubler, D.J. , Ooi, E.E., Vasudevan, S.G., and Farrar, J. (Editors).  CABI, New York. 2014.
CABI bookshop          Amazon.com
Joanne L. Tan and Shee-Mei Lok. (2014). Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy. In: Padmanabhan R., Vasudevan S. (eds) Dengue. Methods in Molecular Biology (Methods and Protocols), vol 1138. Humana Press, New York, NY.
Springer Link
updated on 2021-06-04